538 Theophilüs s. Painter, 



similar to that found in Maevia vittata that there is no need of 

 giving- a detailed description here. The material does not afford 

 any spermatogonial stages which prove illuminating, so that this 

 chapter of the spermatogenesis is closed until more material can be 

 obtained. In view of the asymmetrical distribution of the ctetosomes 

 in Maevia vittata it would be very interesting to determine if we 

 have the same thing in Amaurobius sylvestris. 



First maturation division. 



In the cells shown in Figs 67 and 68, we have the typical 

 rest period as seen in this form. In the former figure we find two 

 large nucleoli C^A") which unquestionably represent the accessory 

 chromosome elements and, besides these, we find seven smaller deeply 

 staining bodies, marked "P'. The autosomes appear at this time 

 as a very faintly staining reticulum. 



The Ehelich-Biondi-Heidenhain triple stain has been used on 

 this form in order to determine if the nucleoli found at this time 

 were true chromatic structures. This differential stain showed 

 beyond doubt that the larger nucleoli were chromatin and the 

 smaller bodies stained green. The results were not sufficiently clear 

 but in the case of the smaller bodies to be convincing, although 

 they showed the reaction of true chromatin. 



The number of nucleoli seen in the rest period vary within 

 wide limits in different cells. We find generally either two large 

 nucleoli lying in the nuclear cavity with a varying number of smaller 

 bodies, or we find one very large nucleolus as shown in Fig. 68, and 

 a number of smaller bodies. A careful study of the behavior of 

 the chromatin elements shows that these bodies ultimately fuse, as 

 was the case in Maevia vittata. It is very diffucult to determine 

 wüth certainty the number of smaller nucleoli present in the cell 

 because of this fusion. In Fig. 67, seven small nucleoli are seen, 

 while in the cell shown in Fig. 68, nine smaller bodies are seen in 

 addition to the very large nucleolus which represents the fused 

 accessory chromosome elements. Nine small nucleoli is the largest 

 number seen in any cell of the rest period, and this probably is 

 very near the total number which the cells may contain. The great 

 variation which the cells of Fig. 67 and 68- show in the size of the 

 nucleoli has a very simple explanation. The slide from which 

 Fig. 67 was taken, had been extracted a long time in order to make 



