546 Theophilus s. Painter, 



1 cell had 5 Nucleoli 



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This table shows quite clearly that the ultimate distribution of 

 the hétérochromosome elements is very irregular. 



The spermatids retain the condition shown in Fig. 84, for 

 some time. Then there follows a stage when they lose their 

 affinity for the stain and at this period the hétérochromosome 

 elements can not be distinguished except with great difficulty. 

 After this period the sperm head begins to form. 



Number of chromosomes. 



Spermatogonial stages are lacking from my material; so no 

 observations have been made on cells of this period. The primary 

 spermatocyte cells are unfavorable for study as there is much over- 

 lapping of the autosomes. For counts of this form, equatorial plates 

 of the second division have been relied on. Fig. 82 and 83 show 

 typical cells of this division. There are sixteen autosomes present 

 in the equatorial plate at this time. The diploid number of auto- 

 somes would be on this basis as follows: 



Autosomes 32 



Accessory chromosome 2 



Supernumeraries 9 (?) 



Total 43 

 The above account has been based on a study of one specimen 

 preserved in Bourn's liquid. Comparison has been made of the 

 behavior of the chromosomes of this specimen with two other males 

 which were preserved in Petrunkevitch's sublimate. The same 

 behavior of the supernumerary chromosomes was found in all cases. 

 All of the specimens have the ctetosomes but the number of 

 planosomes varies, as we should expect. One specimen showed 

 seven supernumeraries in a telophase stage of the first division, 

 while the other showed very few, only three or four. It should be 

 noted that the three males studied came from an isolated section of 

 woods near New Haven, Conn. 



Spermatogenesis of Spermaphora ^neridionaUti, 



The Fholcidae have given the most unsatisfactory preparation 

 of any of the spiders studied up to the present. The germ cells 



