author's abstract of this paper issued 

 BY THE Bibliographic service, august 7 



DEMONSTRATION OF EPITHELIAL MOVEMENT BY 

 THE USE OF VITAL STAINING, WITH OBSER- 

 VATIONS ON PHAGOCYTOSIS IN THE 

 CORNEAL EPITHELIUM 



SHINICHI MATSUMOTO 



Osboni Zoological Laboratory, Yale University 



FOUR FIGURES 



Though the cornea of the adult frog is thin and transparent 

 enough for the observance of epithelial movement, it is not an 

 easy matter to note in detail every part of the process. For 

 this reason certain vital stains were used in the present 

 experiments. 



In the amphibians, vital staining can be produced either by 

 introducing the dyes into water in which the animals swim, or 

 by injection, though the latter procedure is not practicable in 

 the case of corneal epithelium, owing to its lack of blood-vessels 

 The stained tissue may then be explanted. Another method is 

 to add the dyestuffs to the culture medium containing the un- 

 stained tissue. 



Several papers have appeared dealing with the different phases 

 of this subject (Lewis and Lewis, '15; Russel, '14). 



For the purpose of the present work it is necessary that the 

 dyes should not have any deleterious effect upon the epithelial 

 cells in order that they may remain capable of maintaining their 

 normal activity. The staining must be produced easily and 

 last for a considerable time. 



That the epithelium of amphibians (especially larvae) is 

 tingeable by various dyestuffs without affecting the organism or 

 tissue elements to any marked degree has been shown by various 

 observers (Fischel, '01). WTiether vital staining may be 

 employed in order to demonstrate moving epithelium in the cul- 

 ture is, however, another question. 



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