REACTION OF SELACHII TO INJECTIONS 105 



'1.' Also in every case the anterior portion of the kidney and 

 liver was of a light blue color. The posterior portion of the kid- 

 ney was dark blue and a dissection of the liver showed the bile 

 ducts to be colored blue rather than the usual green. The 

 other organs of the body cavity were not distinctly blue in color. 

 Glands were fixed in absolute alcohol, but the dye soon dis- 

 appeared either by fading or going into solution. Sections were 

 made and examined microscopically, but no blue granules were 

 seen even though the gross specimens were blue. The dye was 

 probably impure and hence dissolved out in the alcohol, as was 

 found to be true in some cases by Heidenhain ('83) working with 

 other animals. 



B. Potassium iodide. An adult male fish received 10 cc. of 

 a 3 per cent solution of potassium iodide intravenously. After 

 three and a half hours tests were made with nitric acid and 

 starch (Hawk, '16), and the iodide was found to be present in 

 the urine in the gastric and intestinal fluids, the bile, and the 

 secretion of the digitiform gland. 



C. Dextrose. An adult female fish received intravenously 

 15 cc. of 25 per cent dextrose solution. At the end of one hour 

 the urine was negative to Benedict's sugar test (Hawk, '16), 

 but was positive after four hours and negative again after nine 

 hours. At the latter time the bile gave a positive reaction, but 

 the secretion of the digitiform gland reacted negatively.' 



D. Sucrose. The animal i-eceived intravenously 15 cc. of 

 30 per cent sucrose, and after six and one-half hours was autop- 

 sied. The reaction to Benedict's test was positive in the case 

 of the urine, bile, and blood serum, but negative with the gastric 

 and spiral-valve contents. The digitiform gland secretion was 

 not tested. 



E. Phenosulphonepthalein (Rowntree and Geraghty, '12). 

 1. One and two-tenths cc. of this pthalein was injected intra- 

 muscularly. It appeared in the urine in fourteen minutes. At 

 autopsy it was not present in detectable amounts in the stom- 

 ach, intestines, or digitiform gland. 



2. An animal received intravenously 1 cc. of pthalein. Au- 

 topsy was performed after one hour. At this time the pthalein 



