106 E. R. HOSKINS AND M. M. HOSKINS 



was present in the urine, bile, and in the stomach and spiral- 

 valve contents. The secretion of the digitiform gland and an 

 extract of the spleen were negative. 



3. An adult fish received 1.4 cc. of pthalein. The urine was 

 collected for five and a half hours and the bile in the gall-bladder 

 was removed at the end of that time. The urine, bile, and 

 1.4 cc. of pthalein in graduated cylinders were each diluted with 

 water, acidified and all compared (Rowntree and Geraghty, '12). 

 By this comparison it was shown that approximately one-half 

 of the injected pthalein had been recovered, and of this, about 

 one-fourth was in the urine and three-fourths in the bile. The 

 bile pigments interfere shghtly with the test. A trace of ptha- 

 lein was found in the serum, digitiform gland secretion, and in 

 the stomach and spiral-valve contents. That in the latter two 

 may have come from excreted bile. 



4. An adult received 1 cc. of pthalein. A cannula was in- 

 serted to collect the urine and the animal was then permitted 

 to swim about in the aquarium for four and a half hours in 

 order that the excretion might not in any way be interfered with 

 by the unnatural position of the animal in the holding apparatus 

 that was used in the first three experiments. In this specimen 

 as in the others more pthalein was recovered in the bile than in 

 the urine. Pthalein was also found in the spiral valve, but not 

 in the stomach. 



F. Weed's solution (potassium ferrocyanide and iron ammo- 

 nium citrate, Weed, '12). 1. A young male (0.8 kg.) received 

 intra muscularly 7.5 cc. of Weed's solution and was autopsied 

 after four hours. The urine and bile gave the Prussian-blue 

 reaction with acid. The serum and the stomach and spiral- 

 valve contents were negative to the test. 



2. A young animal (0.5 kg.) received intramuscularly 9 cc. 

 of Weed's solution, followed after five hours by 9 cc, and after 

 twenty-two hours by 8 cc. Twenty-five hours after the first 

 injection the Prussian-blue reaction was obtained with the 

 urine, bile, and serum. The liver turned blue on standing. 



3. A young female (0.5 kg.) received, as above, 10 cc. of the 

 solution, followed by 5 cc. after four hours, and 5 cc. after seven 



