108 E. R. HOSKINS AND M. M. HOSKINS 



the hepatic sinuses have a deposit of blue granules on or in them. 

 This condition is not seen in other organs of the same animal" 

 nor in the liver of other animals. 



d. Spleen. Blue granules are scattered throughout the spleen 

 among, but not within the cells. 



e. Spiral-valve, stomach, gills, and muscle. All these have 

 blue granules in the blood-vessels and scattered through the 

 connective tissue, but none within any cells. In gross free- 

 hand sections the epithelium of the stomach and spiral-valve 

 appears white, in striking contrast with the dark blue connec- 

 tive-tissue. 



2. Suspensions 



A. Carmine. Powdered carmine was used for coarse granules, 

 although some of it seems to go into solution in sea-water. It 

 will not all settle out on standing, cannot be separated out with 

 ordinary filter-paper, and will even pass through a celloidin 

 membrane. After passing through celloidin no granules can be 

 seen with the ordinary microscope. This may not be in true 

 solution, but if not it is practically so.- 



1. Filtered carmine after setthng for several days was injected 

 intravenously into an adult male dogfish. The animal received 

 four daily doses of 12 cc. each and was, autopsied eight hours 

 after the last injection. Microscopic examination of the various 

 organs failed to show the presence of any carmine granules, 

 although the spleen appeared distinctly red after fixation. The 

 granules were either too small and too much scattered to be 

 seen or else the carmine was in solution and washed out in pre- 

 paring the sections. The urine was not collected. 



2. Ten adult animals were injected intravenously each with 

 10 cc. of a heavy suspension of powdered carmine in sea- water 

 and autopsied after various periods as follows: 1|, 6|, 21, 24, 

 50, and 96 hours. This carmine was not filtered. Pieces of the 

 various organs and tissues were fixed in formahn, sectioned in 

 paraffin, and stained with haematoxyUn, or examined unstained. 



Urine was collected from -several of the animals, but in only 

 one specimen of it was any carmine found, and in that but one 



