KEACTION OF SELACHII TO INJECTIONS 111 



1. An adult received intravenously. 10 cc. of suspension of 

 neutral red and was permitted to swim about in the aquarium. 

 At that time the animal was immobilized, received an addi- 

 tional 18 cc. of the suspension, and its urine was collected for 

 five hours without any of the neutral red appearing in it. Au- 

 topsy showed the bile to be reddish brown. This turned orange 

 with alkali and dark lilac with acid. Serum gave a similar 

 reaction, but the gastric fluid was negative. The suspension 

 was prepared by stirring neutral red in sea-water and allowing 

 the mixture to settle. 



2. An adult was injected intravenously with 15 cc. of satu- 

 rated neutral red suspension. The urine was collected for six 

 hours and was at all times free of the dye. Autopsy showed 

 neutral red in the bile in a considerable amount. 



3. A female received intravenously, 10 cc. of the neutral red 

 suspension and the urine was collected for eight and a half 

 hours without any of the dye appearing in it. As above, it was 

 present in the bile. 



The two following experiments were carried out a year later 

 than the former, with a different sample of neutral red. This 

 could not all be removed by filtering and part of it even passed 

 through a celloidin sac, so that it probably was partly in 

 solution. 



4. Ten cc. of filtered neutral red in sea-water was injected 

 intravenously into an adult fish. Four hours later, examination 

 showed the dye to be in both the bile and urine, but not in the 

 stomach or spiral-valve contents. 



5. Eight cc. of the dye in the above form was injected intra- 

 venously into an adult dogfish. There was 24 cc. of bright red 

 urine collected in twenty-four hours, and at autopsy 2.5 cc of 

 reddish-brown bile was removed from the gall-bladder. The 

 24 cc. of urine was diluted to 100 cc. with water and the 2.5 cc. 

 of bile to 200 cc. Maximum redness was produced by neutral- 

 izing, and the color of the two were then of about the same 

 intensity. Thus the total excretion of the dye by the fiver was 

 more than eighty times as much as that excreted by the kidney. 

 The total amount of dye removed by the liver was not collected 



