REACTION OF SELACHII TO INJECTIONS 121 



numerous eosinophilic leucocytes. In sections of the spiral 

 valve stained together with sections of a normal spiral valve the 

 chromatin in the epithelial cells of the former which appear 

 otherwise normal in structure stains much less darkly with 

 haematoxyhn than does that of the normal tissue. 



g. Stomach. The stomach is in about the same condition as 

 in the 'chromate' specimen. There are present in the blood- 

 vessels and lamina propria numerous eosinophilic leucocytes. 



C. Uranium nitrate. 1. An adult received intramuscularly 

 90 mg. per kg. body weight of uranium nitrate in 1 per cent solu- 

 tion. It lived forty-four hours and was then autopsied at once. 

 The spiral valve was greatly congested, but the other organs 

 appeared normal. 



2. A male of 1 kg. received 100 mg. of uranium nitrate as 

 above. Respiration ceased after two hours, but was restored by 

 an hour of artificial respiration produced by inserting a tube of 

 running water into the animal's mouth. The fish died after 

 twenty-eight and a half hours and was immediately autopsied. 

 The spiral valve contained bloody fluid and its mucosa was con- 

 gested. A few small hemorrhages appeared in the kidney. 

 No other abnormalities were noted. 



3. An adult of 1 kg. received intramuscularly 100 mg. of 

 uranium nitrate. A cannula was inserted into the bile duct to 

 prevent any toxin that might be in the bile from reaching the 

 spiral valve. After five hours the urine was tested and found to 

 contain uranium nitrate. The next morning the animal received 

 an additional 90 mg. of the toxin. At 8 p.m. it was still active, 

 but died in the early morning, about forty hours after the first 

 injection. The body was not yet rigid at 8 a.m., so tissues 

 were fixed for microscopical examination, as few postmortem 

 changes had had time to occur. This was confirmed by the 

 microscopical examination. The kidneys were congested, but 

 other organs appeared normal. 



4. Eight dogfish of various sizes were injected with 100 mg. to 

 200 mg. per kg. body weight, of uranium nitrate. A cannula 

 was inserted into the bile duct to protect the spiral valve from 

 toxin in the bile. The urine was collected. Uranium nitrate 



