GROWTH AND STRUCTURE OF HYPOPHYSIS IN RAT 



325 



TABLE 1— Concluded 



NOSE-ANUS 

 LENGTH 



BODY WEIGHT HYPOPHYSIS 



GROSS (and net) weight (fresh) 



E 2. Rats rejed one xoeek after maintenance {three to twelve weeks) 



E 4- Rats refed four iveeks after maintenance {three to twelve weeks) 



109 

 109 

 109 

 109 

 110 

 111 



171 

 161 

 151 

 163 



167 

 168 



125.2(117.4) 

 106. 5( 98.0) 

 94. 2( 88.4) 

 118.0(110.1) 

 118.0(109.5) 

 129.5(119.1) 



0.0056 

 0.0082 

 0.0058 

 0.0088 

 0.0068 

 0.0094 



The material was obtained immediately after the animals were 

 killed (by chloroform). Especial care must be taken to avoid 

 injury in the removal of delicate organs like the hypophysis. 

 The glands were in nearly all cases fixed in Zenker's fluid and 

 stained with haematoxylin-eosin. Formalin and Zenker-formol 

 were tried as fixatives, and iron-haematoxylin as a stain, with 

 less satisfactory results. The glands were embedded in paraffin, 

 and cut in sections (usually serial) 3 ^i to 5 ^ in thickness. The 

 sections were cut in the frontal (coronal) plane. 



