350 C. M. JACKSON 



A\ ith a corresponding diminishing intensity of the specific stain. 

 In some regions (especially in the central portion of the gland) 

 the staining reactions may be preserved much better than else- 

 where. 



Corresponding to the cytoplasmic atrophy, there are also 

 changes in the nucleus. That there is loss in volmne (though 

 less than in the cytoplasm) in the nucleus has already been shown 

 by measurements. In structure, some areas may show rela- 

 tively little nuclear change, but in the great majority of cases 

 there is a very decided hyperchromatism (figs. 3 and 5). This 

 is best marked in the peripheral atrophic regions above referred 

 to, where all the nuclei may present the typical pycnotic condi- 

 tion, shrunken, deeply staining and homogeneous. Or inter- 

 mediate stages may occur, in which the nuclei are larger, with 

 the chromatin dissolved into a pale-blue homogeneous matrix 

 which obscures the nuclear network. Karyorrhexis and karyoly- 

 sis also occur, but are comparatively rare. 



In cases or areas where the atrophic changes are not so far 

 advanced, it appears that the pycnosis first becomes evident in 

 the nuclei of the eosinophiles. This might be expected since, as 

 above stated, hyperchromatism and even occasional pycnosis 

 occur in the strongly chromophilic cells of the normal gland. In 

 some cases during inanition it appears also that the acidophilic 

 reaction of the karyolymph may persist longer in the nucleus 

 than in the cytoplasm of the eosinophiles. This may be due to 

 physical rather than chemical conditions, however. 



d. Changes during refeeding after maintenance 



Associated with the mitoses already noted in the hypophysis 

 upon refeeding after maintenance, changes take place in the 

 cells leading to a restoration of the normal structure, at least in 

 the greater part of the gland. 



After one-half week of refeeding, although the gland has in- 

 creased appreciably in weight, and mitosis has begun in the pars 

 anterior, the cell structure typical for inanition still persists to a 

 very large degree. The only changes noted are a lessening of the 

 hyperemia in the pars anterior, and some increase in the amount 



