282 
Und jetzt mag es mir erlaubt sein, Herrn Prof. Frirscn, in 
dessen Laboratorium ich diese Arbeit vollfiihrt habe, und dem Privat- 
dozenten Herrn Dr. Benpa, unter dessen Leitung ich gearbeitet habe, 
meinen herzlichsten Dank abzustatten. 
Nachdruck verboten. 
Fibres connecting the Central Nervous System and Chorda 
in Amphioxus. 
By Jurıa B. Pıart. 
Aus dem anatomischen Institut der Universität Freiburg i. B. 
Mit 3 Abbildungen. 
Since MÜLLER described two longitudinal rows of openings per- 
forating the dorso-lateral region of the notochord sheath, several ob- 
servers (RoLPH, ROHON, LWOFF, SCHNEIDER and others) have dis- 
cussed and figured fibres passing through these openings from the 
central nervous system to the chorda. 
The existence of such fibres is indisputable. Their nature and 
function are still obscure. Almost unanimously they have been pro- 
nounced non-nervous, and they have been by some observers classed 
with the connective tissues. The following facts in regard to them 
seem therefore worthy of note. 
When Amphioxus is fixed with gold-chloride the fibres show the 
same dark differentiation that distinguishes nerve-fibres when treated 
in like manner. 
If sections of Amphioxus be doubly stained by Dr. C. Röse’s ') 
method with alum-carmine and bleu de Lyon, in which connective 
tissues appear intensely blue, while the remaining tissues maintain the 
purple shade of alum-carmine, they show the sheath of the notochord 
stained deep-blue and broken by a series of openings containing fibres 
that have not been affected by the blue stain, and which are therefore 
presumably not connective tissue fibres. 
Owing to the delicacy of the fibres I am unable to trace them in 
cross-sections of 1/,,, mm from their origin in the central nervous 
1) Entwickelung der Zähne des Menschen. Archiv f. mikr. Anat., 
Bd. 38, 1891, p. 448. 
