122 H. E. JORDAN 



mitochondria are discernible in the granulocytes. Nor are the 

 several varieties of specific granules preserved. These granules 

 of granulocytes are represented, with few exceptions, only by 

 vacuoles, the negatives of the dissolved granules. In certain 

 other sets, prepared by the same technic, the hemoblasts and 

 giant-cells, with rare exceptions, lack mitochondria; but in 

 these cases the granules of the granulocytes are deeply stained. 

 They are spherical and annular in shape (fig. 13), and appear 

 identical with the mitochondria of the giant-cells of the other 

 sets of preparations. They can, however, be definitely identified 

 as non-mitochondrial granules by other than morphologic and 

 tinctorial criteria, and not the slightest confusion exists betw^een 

 the cytoplasmic granules of blood granulocytes and the mito- 

 chondria of hemoblasts and giant-cells. In certain sets only 

 the former are preserved, in others only the latter. The point 

 of special importance here concerns only the fact that even the 

 Kopsch technic is not specific for mitochondria. This technic 

 stains in the same way also the specific granules of the amphophil 

 (special) and eosinophilic granulocytes, and in such manner as 

 to closely simulate mitochondria. 



The large eosinophilic granules stain generally only a deep 

 brown (fig. 13 b), while the granules of the special leucocytes 

 stain black (fig. 13 a). Error and confusion are obviated by the 

 fact that only one of the elements, that is either the specific 

 granules or the mitochondria, stain, with an occasional excep- 

 tion, in the same preparation. This fact emphasizes also the 

 extreme capriciouness of the action of this most favorable mito- 

 chondrial technic. Moreover, the reaction to osmic acid suggests 

 that the amphophil and eosinophil granules also contain a lipoid 

 constituent. The ring-shaped character of especially the eosino- 

 philic granules results most probably from the solution of the 

 central, less resistant, portion of the granules by this technic, 

 or it may perhaps result from the rarefaction of the center of the 

 granule due to a swelling action of the osmic acid. 



In hemoblasts appear only relatively few mitochondria, and 

 a large number of these are of the short bacillary variety (figs. 

 1 to 4) . In general, as the giant-cell progresses in development, 



