126 H. E. JORDAN 



cells.' This conclusion he regards as favored also by the fact 

 that the canaliculi open upon the periphery. 



It seems important to record the fact that the appearance of 

 the cytoplasm differs greatly in different cells of my preparations 

 fixed with Carnoy's fluid. In some cells it appears homogeneous 

 or very finely granular, without indication of either canalicular 

 network or mitochondria; in other cells the cytoplasm is filled 

 with black-staining granules and varicose threads, the granules 

 being apparently identical with the mitochondria of the Kopsch 

 preparations. In the cells in which the cytoplasm is canalized 

 as above described, the black-staining granules and threads 

 are either extremely rare or entirely lacking. I am unable 

 to decide, on the basis of available data, whether this difference 

 in the constitution of the cytoplasm represents a genuine func- 

 tional and structural difference or a variable effect of the fixing 

 process, or whether it is correlated with a difference in the stage 

 of development, showing itself in a difference of reaction to the 

 Carnoy mixture. The latter interpretation seems to me the most 

 probable. 



It is of prime interest that Retzius states that he was unable 

 to demonstrate 'this canalicular apparatus of giant-cells by 

 the application of Golgi's chrom-osmium-silver method so 

 favorable for revealing this apparatus in gland cells.' This was 

 also my experience. In fact, I was unable to demonstrate such 

 a canalicular apparatus of Holmgren or a genuine Golgi reticulum 

 by any of the standard methods, other than the Kopsch method 

 in the case of the Golgi net, in general use for the preservation 

 of these cell contents. 



In my experiments with various fluids for the demonstration 

 of mitochondria and the Golgi apparatus in the giant-cells of 

 bone-marrow I used in practically every attempt also testicular 

 tissue. With the Kopsch method it was a sijnple matter to 

 demonstrate in the same sections of the testis (rabbit and opos- 

 sum) structures identical with what have been described and 

 illustrated many times by many workers with various technics,, 

 both mitochondria and Golgi apparatus (fig. 14). With the 

 time modification the Kopsch method revealed similar structures 



