128 H. E. JORDAN 



nucleated conditions of these giant-cells. But the Golgi appa- 

 ratus clearly enlarges and becomes more of the nature of a closed 

 network, always located close to the area which contains, as 

 deduced from other preparations, the fragmenting centrosomes 

 (fig. 5). In the later polynucleated stages, determined as 

 accurately as the poorly preserved nuclei of this technic will 

 permit, the reticular apparatus is lacking. It apparently breaks 

 up and disappears coincidently with the partition and disap- 

 pearance of the centrosomes (fig. 9) . 



The question of relationship between Golgi apparatus and 

 mitochondria, seeing that they react in similar fashion to the 

 osmic acid, presents itself for consideration. On the basis of 

 staining reaction in osmic acid, one might conclude that Golgi 

 apparatus and mitochondria are only different portions or 

 different morphologic expressions of a chemically identical, 

 certainly similar, cytoplasmic element. Such conclusion would 

 seem to receive support from the fact that, when closely analyzed, 

 the Golgi apparatus, especially in male germ cells, appears to 

 be composed of elementary rods and granules, fused into a more 

 or less intricate and continuous network; and further, from the 

 additional fact that the mitochondria tend to aggregate about 

 the centrosphere, the vicinity of which is frequently the site 

 also of the Golgi apparatus (fig. 14). 



In the hemoblasts and their giant-cell derivatives the Golgi 

 apparatus is located close to the position of the centrosome 

 (figs. 1 to 4) . If the mitochondria which have congregated about 

 the centrosphere fused, a network would presumably result very 

 similar to the network of this location called the Golgi apparatus 

 (fig. 5). However, there is no crucial evidence to prove that 

 the granules scattered throughout the cytoplasm, and which 

 appear identical with mitochondria, are not actually derived by 

 fragmentation from the Golgi apparatus. Determination of 

 relation, on the basis of staining reaction to osmic acid, is made 

 to appear still more uncertain when we recall the close similarity, 

 amounting apparently to an identity, between the special (ampho- 

 phil) and eosinophilic granules of the granulocytes of this marrow 

 and the mitochondria of the giant-cells. 



