176 JOHN STEPHENS LATTA 



and staining reactions are identical with the 'tingible Korper' 

 as described by Flemming. 



The nature of the inclusions, if they are such, and the origin, 

 fate, etc., of these acid-staining cells have not been satisfactorily 

 determined. If these deeply staining bodies are foreign bodies, 

 phagocytized by the non-granular acidophile cells, what, then, 

 are the foreign bodies derived from? 



If these bodies are inclusions and the cells containing them 

 phagocytic, the cells should then take up particles of colloidal 

 dyes if solutions of the dye are injected into the animal's body. 



In order to determine whether the acid-staining cells possessed 

 any phagocytic power or not, solutions of the acid azo colloidal 

 dye, trypan blue, were used. Large quantities of a 5 per cent 

 aqueous solution of the dye were injected into the peritoneal 

 cavity of rabbits. In these instances all the dye was phagocy- 

 tized by the large free cells in the peritoneal cavity and cells 

 of the subperitoneal connective tissue. In order, then, to get the 

 dye in a position where it could be acted upon by cells in the 

 intestinal lymphatic nodules, operations were performed in which 

 the peritoneal cavity was opened and a 5 per cent aqueous solu- 

 tion of the dye injected directly into the lymphatic tissue of 

 Peyer's patch and the appendix, and the wound sewed up. 

 After twenty-four to forty-eight hours the animals were killed 

 and the tissue fixed in the usual way. 



Examination of this tissue revealed the fact that large quanti- 

 ties of the dye had been phagocytized by the acid-staining cells 

 and by the connective-tissue cells outside the nodules, the lym- 

 phocytes being free of it, except immediately about the point 

 of injection, where they were diffusely stained, the cells at this 

 point being apparently killed by the shock of injection. This 

 shows clearly that these cells possess phagocytic powers .and 

 belong to the group of cells called macrophages (Evans) (fig. 6). 



As to the nature of the inclusions, or Hingible Korper,' 

 found in these cells, it might be assumed from their appearance 

 and staining reaction that they were nuclei of degenerated small 

 lymphocytes in this region, and such, indeed, is the case. The 

 'tingible Korper' of Flemming, then, are remnants of nuclei 



