LIVER CELLS FROM CHICK EMBRYO " 289 



green. In one case most of the mass faded to a dark green except 

 one small spot which remained red and another small spot brown. 

 When trypan blue cultures were treated with potassium per- 

 manganate neither the green color of Ihe bile masses nor the blue 

 color of the stained cytoplasmic granules had shown any change 

 three and a half hours later, when the observations were dis- 

 continued. Similar bright green granules and masses were found 

 in the mesenchymal cells of a few cultures made from the gall- 

 bladder of a nine-day embryo. The explants and the debris in 

 these cultures were also bright green. 



Other cytoplasmic granules 



Other granules were found in both the endoplasm and ecto- 

 plasm and also in vacuoles when these were present. Many of 

 them seemed to lie in the transition region between the ectoplasm 

 and endoplasm where they moved about very actively. These 

 bodies were of various shapes and easily distijiguishable from the 

 mitochondria by their different refractivity, by their rough 

 irregular contour, and by their affinity for neutral red and trypan 

 blue (figs. 9, 10, 11, 12, 13, 14, 17, 18). They varied in size from 

 granules barely visible with the highest magnifications to masses 

 as large as the nucleolus and in a few cases as large as the nucleus. 

 Their form was very different from that of the corresponding 

 granules found in the mesenchyme cells, being peculiarly angular 

 and irregular. The smaller granules moved about very actively, 

 often passing swiftly along the entire edge of the cells, or the 

 full length of their enclosure. For this reason they were easily 

 seen without staining. The larger bodies moved less rapidly 

 and less extensively. These granules were observed in cultures 

 from embryos of from five to twelve days' incubation. In a few 

 cultures they were not present in the first cells which projected 

 from the edge of the explant, but in most cases they were found 

 in all stages of cultivation from the appearance of the first migrat- 

 ing cell to the death of the culture, in some instances as long as 

 nine days after planting. In early stages of incubation the 

 granules were few and small, increasing in both number and size 

 as cultivation progressed until, in cultures four or more days old, 

 they were often large and numerous (figs. 11, 17). 



