292 RUTH STOCKING LYNCH 



rose-red, very different from the yellow-red color they exhibited 

 when stained with neutral red alone. These color changes 

 occurred first in the cells at the very edge of the growth and 

 progressed inward toward the explant, which finally became 

 tinged with the neutral red, usually, however, not going beyond 

 the purple stage. The granules in all the cells of the culture 

 showed these color changes. The granules in the endothelial 

 cells showed the same degrees of staining with the two dyes. 

 The wandering cells usually took up more of the neutral red than 

 the other cells, their granules being rose-red when the granules 

 of the other cells w^ere lavender, and lavender w^hen the others 

 were purple. 



When the cells of such doubly-stained cultures died, the neutral 

 red color disappeared first, the trypan blue later, so that in a 

 degenerating culture there might be at the same time cells show- 

 ing lavender granules, others showing blue granules, and debris 

 which was colorless. Much the same effect was obtained when 

 the cultures were treated with potassium permanganate. Blebs 

 often appeared very soon after the permanganate was added to 

 the culture, and the red color was gradually lost, sometimes being 

 entirely gone after two hours, at which time the blue staining 

 had not usually been affected. Often it was not possible to recog- 

 nize colorless granules after the neutral red or trypan blue had 

 disappeared from the cell, probably owing either to their dis- 

 integration or to the generally contracted, degenerate condition 

 of the cell. 



The distribution of these cytoplasmic granules was not at all 

 constant. Although most young cultures showed them, not all 

 did; nor did all the cells of a culture behave similarly in this 

 respect. Frequently one liver cell would show granules while a 

 neighboring cell showed none at all (figs. 12a, 12b). When 

 vacuoles were present in the cells some of them always contained 

 one or more granules in • active Brownian movement, while in 

 some vacuoles no granules were visible (figs. 2, 9, 10, 11, 17a, 17b). 

 In the marginal cells the greater number of the granules usually 

 lay in the distal portion of the cell (fig. 9), but this was probably 

 merely the result of the position of the nucleus which was usually 



