LIVER CELLS FROM CHICK EMBRYO 297 



The granules in these cells showed the same color reactions 

 as those of the liver cells, except that the colorless ones took the 

 stains much more deeply, making the wandering cells the most 

 prominent features of the cultures. The granules stained an 

 intense red with neutral red, a deep blue with trypan blue, and 

 a good purple or lavender when both dyes were present in the 

 culture medium. They took up neutral red more readily than 

 trypan blue when both were present, so that when the other cells 

 had lavender granules the wandering cells had rose-red granules, 

 or lavender granules when the other cells had purple ones. They 

 were the last cells to lose their staining when the culture de- 

 generated. 



The green bile masses in the cells stained slowly but intensely 

 with the neutral red, but apparently they did not take up the 

 trypan blue. In trypan blue cultures both blue and green gran- 

 ules were present in the same wandering cell. In neutral red 

 cultures all the granules were stained red. 



These cells are much more satisfactory for study when stained 

 with trypan blue than when stained with neutral red, as the 

 granules when stained with the latter dye mask the fat globules 

 and the nucleus to a much greater extent than when stained with 

 the blue. 



DEBRIS 



In many liver cultures a large amount of debris accumulated, 

 sometimes entirely obscuring the details of the growth. This 

 debris occurred either as groups of long anastomosing threads 

 stretching out from the edges of the explant and finally over- 

 growing the whole field, or as a fine granular or flocculent 

 precipitate deposited all over the field. The long, rough, inter- 

 laced threads often had the granular debris caught in among 

 them; sometimes long, single, isolated threads stretched out 

 from the explant to a considerable distance. The two types 

 stained alike, taking hemateine, hematoxylin and Gram's stain 

 very deeply. In no other kind of cultures made in this laboratory 

 has debris of this sort occurred so frequently or in such large 

 amounts as in these liver cultures. 



THE AMERICAN JOURNAL OF ANATOMY, VOL. 29, NO. 2 



