8 MARY JANE HOGUE 



without taking it in. The granules could lie in any position in 

 relation to the advancing pseudopodium. They were horizontal 

 and perpendicular to it and at times formed an angle with it. 

 Often they were swung around from a perpendicular to a horizon- 

 tal position and vice versa, and sometimes they swung from a 

 perpendicular or horizontal position to a slanting one and \'ice 

 versa as they entered the ectoplasm. Once in the ectoplasm 

 their position was constant until they reached the endoplasm. 

 As soon as they were there, they were whirled about by the endo- 

 plasmic currents. 



As a rule, only one granule was taken in at a time, though I 

 have seen a group of three go in at once, and frequently two gran- 

 ules will be taken in at different parts of the pseudopodium at the 

 same time. The amoeba is capable of taking in a large number 

 of these granules. Four hours after the pigment granules were 

 introduced into the hanging drop of amoebae, the amoebae were 

 filled with the granules. They had at least a hundred granules 

 and resembled the pigment cells of the retina, except for the fact 

 that they were moving rapidly. 



The amoeba takes the granules in by simply pushing against 

 the granules with such force that the granule pierces the ecto- 

 plasm. For this reason the granule to be taken in must be in 

 the path of the advancing amoeba. If it is to one side of the 

 anterior pseudopodium it is passed by; if it is near the side of 

 the pseudopodium, but still anterior to it, it may be taken in or 

 it may be pushed off farther to the side, depending on how far to 

 the side it is and also on how rapidly the amoeba is moving. If 

 it is moving very fast the granules near the outer edge of the 

 anterior pseudopodium are much more likely to be pushed away 

 than to be taken in, or they may remain attached to the amoeba 

 like the spines of a sea-urchin and be passed back along the edge 

 until they reach the posterior end. Here they frequently remain 

 attached for a long time, but are never taken in at this point. 

 When the amoebae have been in a medium full of these melanin 

 granules for some time many of them are found carrying at least 

 fifty or more granules on their pointed posterior ends. They 

 fairly bristle with the pigment granules at this point. 



