author's abstract of this paper issued 

 by the bibliographic service, december 12 



A COMPARISON OF AN AMOEBA, VAHLKAMPFIA 

 PATUXENT, WITH TISSUE-CULTURE CELLS 



MARY JANE HOGUE 



Department of Medical Zoology, School of Hygiene and Public Health, Johns 



Hopkins University, and the Department of Embryology of Carnegie 



Institution of Washington 



THREE FIGURES 



INTRODUCTION 



While working with tissue cells grown in culture medium I 

 was much impressed with the similarity of their appearance to 

 that of amoebae grown on agar-agar plates, and determined to 

 make a comparative study of these two kinds of cells. The 

 particular cells used for this study were the fibroblasts and the 

 white blood cells from the embryonic chick and an amoeba, 

 Vahlkampfia patuxent (Hogue, '21), which is a salt-water form, 

 parasitic in the digestive tract of the oyster. 



The tissue cells were grown at 39° in hanging drops of Locke- 

 Lewis solution (Lewis and Lewis, '15) inverted over hard vaselin 

 rings on depression slides.^ The amoebae were taken from agar- 

 agar plates made up with 0,7 per cent sodium chloride solution, 

 with 0.4 per cent peptone, and kept at room temperature. 



The comparison of these two kinds of cells was made by study- 

 ing permanently stained preparations, by introducing the amoebae 

 into the cultures where the tissue cells were growing and here 

 studying their different reactions to various vital stains and 

 pigment granules and by further testing out the effect of these 

 stains and pigment granules on the different kinds of cells in their 

 own culture media. 



1 Mrs. Lewis kindly furnished me with tissue cultures of connective tissue and 

 spleen. 



1 



