72 DWIGHT E. MINNICH 



intensity, as indicated by the total amount of response pro- 

 duced, and, third, time aspects, viz., latent period and period of 

 execution. 



Let us take first the response to distilled water. This response 

 was characterized by its close relationship to the nutritional 

 condition of the animal. As shown above, in the experiments on 

 stimulation of individual tarsi, table 1, the responsiveness to 

 water could be increased or decreased by preventing or allowing 

 access to water. The same results were obtained when all four 

 tarsi were stimulated simultaneously. The curves of response to 

 water in figure 3 show in every instance that with continued 

 starvation the response finally rose to 100 per cent, while directly 

 after water was administered it dropped to per cent, where it 

 generally remained as long as water or an aqueous solution was 

 accessible. In other words, the response to water depended 

 directly on the nutritional state of the animal. In this respect 

 it was absolutely unique, for in no other response was any intel- 

 ligible relationship to nutritional condition evident. The re- 

 sponse to distilled water is thus sharply differentiated at the out- 

 set from the other responses studied. Consequently, we may 

 omit it from further consideration, confining our attention solely 

 to a comparison of the responses to the three solutions. • 



A survey of the curves of response to IM saccharose (fig. 3) 

 shows that each of the four butterflies responded to this solution 

 in every trial, irrespective of nutritional condition. My notes 

 show that during periods of inanition the response often became 

 more persistent, lasting for some minutes after the trial had been 

 completed. But even during periods when IM saccharose was 

 being fed twice a day, there was never a single failure to respond. 

 Thus, a butterfly which had ceased to feed and had crept away, 

 if replaced on the cotton soaked with the solution, immediately 

 responded anew. And, if after ceasing to feed, the specimen 

 remained on the cotton without creeping away, mere seizure of 

 the wings or other slight agitation was usually sufficient to induce 

 a fresh response. The response to IM saccharose was thus 

 entirely independent of the varying nutritional conditions of the 

 animal. 



