TEANSPLANTATION OF LIMBS IN AMBLYSTOMA 



127 



All of the operated animals were kept under daily observation 

 during the first twenty days. A large number in which the limb 

 and the gills showed marked abnormalities were fixed and only 

 the more important cases were kept. Serial transverse sections 

 were made of eight cases ranging in age from twenty-six to sixty 

 days after the operation. The sections were cut 10 n thick and 

 stained with Ehrlich's haematoxylin and erythrosin. 



4 5 



Fig. 4 Graphic reconstruction of the normal left brachial plexus of Ambly- 

 stoma larva AS426, preserved sixty-eight days after the operation. X "20. (Det- 

 wiler, '20, fig. 13.) 



Fig. 5 Graphic reconstruction of the right brachial plexus of case AS426, 

 showing the segmental nerve supply to the right anterior limb when transplanted 

 the distance of four segments posterior to the normal position. X 20. (Detwiler, 

 '20, fig. 10.) 



Description of cases 



Case AA2Si.rs. This experiment was carried out under the 

 conditions described in table 1 A. There was considerable delay 

 in the growth of the limb bud. Only the first and second gills 

 developed, both of which were rather smaller than normal. At 

 fifteen days after the operation the limb rudiment, which oc- 

 cupied the position of the third external gill, took on the charac- 



