STUDIES ON CYTOLYSINS — SPERMATOTOXINS 209 



February 5th, 14th, and 20th, respectively, in 1920, with the 

 semen derived each time from three male rabbits, after the semen 

 had had sufficient normal salt solution added to it to make a 

 total quantity of 6 cc. This made a dosage of 2 cc. per fowl. 

 Treatment of the two males with the sensitized fowl-serum was 

 begun on March 6th. Both were first examined to see that 

 their spermatozoa were normal in quantity and activity. The 

 untagged male was given the following quantities of this serum 

 on the dates indicated: March 6th, 3 cc. plus 1 cc. Locke's 

 solution; March 8th, 3 cc; March 11th, 4 cc; March 13th, 4.5 

 cc; March 15th, 4 cc On March 3rd, when he was tested, his 

 spermatozoa seemed normal in quantity and activity, and a 

 female to which he was bred on March 13th bore six young. 

 He had become very emaciated during later treatment and 

 died March 18th before further tests could be made. 



The dosage and dates of treatment of male 4A7 with the sensi- 

 tized fowl-serum were as follows: March 6th, 3 cc. plus 1 cc. 

 Locke's solution; March 8th, 3 cc; March 11th, 4 cc; March 

 13th, 6 cc; March 15th, 4 cc After the first injection (March 

 6th) he was mated in order to clear out all spermatozoa that 

 might be in ducts outside the testes. On March 18th, three days 

 after the fifth injection of sensitized serum, his spermatozoa were 

 examined under the microscope. While they were less plentiful 

 than under normal conditions, there was considerable activity 

 among them. However, many immobile ones were visible. 

 Female 80, to which he was mated on this date, bore seven 

 young. His spermatozoa were again examined on March 19th. 

 This time they were still fewer in numbers and most of them 

 were immobile. However, 52A1, mated to him on this date, 

 bore eight young April 21st. Four young were born by 55A4, 

 bred to him on March 20th. His seminal fluid examined on 

 March 23rd was normal in quantity and still had the same milky 

 look as normal semen, but comparatively few spermatozoa were 

 to be seen in the field under the microscope, and practically all 

 of these were immobile. A search for fifteen minutes revealed 

 only two spermatozoa in motion, and these showed only feeble 

 undulations of the tail. Bred to 30A3 on this date, he showed 



