STUDIES ON CYTOLYSINS — SPERMATOTOXINS 213 



normal when compared with the normal control, a much greater 

 proportion of them were active than at the last examination. A 

 mating to female 52A5 on October 6th yielded four j^oung. 



The spermatozoa of the other male, 83, were found to be 

 normally plentiful and active when he was mated to 8A8 on 

 May 13th. He was given intravenous injections of the sensi- 

 tized fowl serum as follows: May loth, 3.5 cc; May 17th, 

 4 cc; May 20th, 4.5 cc; May 26th, 4.5 cc; May 28th, 4.5 cc 

 On May 22nd, microscopic examination showed his spermatozoa 

 to be about normal in number, though many immobile ones were 

 to be seen. A mating to 32A3 on this date yielded seven young. 

 On June 12th he was mated to 23B3 and six young were born 

 July 14, one of which had both hind legs paralyzed. The semen 

 of male 83 on June 12th was normal in quantity, though the num- 

 ber of spermatozoa was noticeably reduced. Many of the 

 latter, however, were active. Conditions were about the same 

 on July 3rd. A mating to 55A4 on this date resulted in thel^irth 

 of eight young. A mating to 43 on October 8th yielded no 

 young, although an examination of the semen still revealed 

 some active spermatozoa. 



A control male (untagged) was injected with normal (i.e., 

 not sensitized) fowl-serum as follows: ^lay 15th, 4 cc; May 

 17th, 4 cc; May 20th, 5 cc; May 22nd, 4 cc; May 28th, 5 cc 

 His semen, examined microscopically May 22nd, June 12th, 

 and July 10th showed no diminution in numbers of spermatozoa 

 nor in their motility. Matings on these dates resulted in litters 

 of five, five, and seven, respectively. 



Tests were also made of the cytotoxic activity in vitro of the 

 respective sera of the four fowls used in this second experiment. 

 The serum of the first fowl (IMay 13th) immobilized the sperma- 

 tozoa of male 25 and male 83 within three minutes. Those of 

 25 were markedly agglutinated, those of 83 showed little or no 

 agglutination. This serum was from the fowl which had re- 

 ceived two intravenous injections of rabbit sperm. The serum 

 of the second fowl (May 17th) immobilized normal spermatozoa 

 within five minutes. Controls in normal fowl serum were stiU 

 moving vigorously thirty minutes later, when observations Avere 



