STUDIES ON CYTOLYSINS — SPERMATOTOXINS 217 



formation or anaphylactic shock could not be determined. 

 November 1st, the three remaining individuals were injected 

 with the semen from three males, and t*his was repeated November 

 8th and November 15th. 



The serum of the sensitized male (untagged) was tested 

 November 22nd. The spermatozoa of this male and those of a 

 normal male were first tested in normal rabbit serum diluted 

 with 3 parts of normal saline solution. In this mixture the 

 spermatozoa of the sensitized male had ceased activity at the 

 end of three hours, while those from the normal male were 

 still very active the next morning, fifteen hours later. It would 

 seem from this that the intravenous injections of spermatozoa 

 which the treated male had received had rendered his spermato- 

 zoa much less viable than those of an untreated rabbit. 



Normal rabbit spermatozoa placed in the serum of the sperm- 

 sensitized male were somewhat reduced in activity at the end of 

 four hours and were wholly inactive next morning, fifteen hours 

 later; normal spermatozoa in normal rabbit serum, used as a 

 control, were still active at the end of twenty-four hours, when 

 observations were discontinued. The spermatozoa of the treated 

 rabbit when placed in his own serum were wholly immobilized 

 by the end of seven minutes. 



Tests made on normal spermatozoa with the blood of the 

 sensitized female, 6A3, gave results similar to thos§ obtained 

 with the serum of the sensitized male. However, the sperma- 

 tozoa of the treated male survived two hours and fifteen minutes 

 in the serum of 6A3 as against seventy minutes in his own. 



A duplicate set of experiments on the serum of this male and 

 female yielded results so nearly identical to the first set that 

 they need not be given in detail. Again the spermatozoa of the 

 treated male survived longer (one hour) in the sensitized serum 

 of the female than they did in his own serum. 



On November 24th similar tests were made with the serum of 

 the same male and with that of the sensitized female, 32A2. 

 This female had borne four young November 18th, and the blood 

 of one of these was also tested. Normal spermatozoa in normal 

 rabbit serum were still in motion at the end of forty-six hours, 



