STUDIES ON CYTOLYSINS — SPERMATOTOXINS 219 



December 26th blood was drawn from 58 and from a normal 

 male and the sera tested. Normal spermatozoa m the serum of 

 58 were completely irmnobilized at the end of twenty-four hours; 

 in the normal serum they were still moving considerably at the 

 end of thirty-two hours, when observations were discontinued. 

 The spermatozoa of 58 when placed in his own serum showed no 

 movement after eleven hours, but in normal serum they showed 

 activity, though very feebly toward the last, for over twenty-four 

 hours. From this test it was evident that rabbit 58 was forming 

 spermatotoxin against his own spermatozoa. 



On January 7, 1921, 58 and 53B2 were each given an injection 

 of his own spermatozoa. About two hours later 53B2 became 

 paralyzed in the hind legs, and as he did not recover he was 

 killed January 17th. Before he was killed his blood serum was 

 tested. Normal spermatozoa in this serum were for the most 

 part inactivated by the end of six hours. In the control of 

 normal spermatozoa in normal serum considerable activity was 

 still in evidence at the end of thirty hours, when observations 

 were discontinued. 



On January 14th rabbit 58 was again injected with his own 

 spermatozoa, and this treatment was repeated on January 21st 

 and 24th, respectively. Thus male 58 received eight intravenous 

 injections of his own spermatozoa in all. His blood-serum and 

 his spermatozoa were both tested February 3rd. Normal 

 spermatozoa in the serum of 58 were mostly immobilized at the 

 end of six hours, although a few maintained feeble movement 

 for twenty-four hours. In the control of normal spermatozoa in 

 normal blood-serum, many of the spermatozoa were fairly active 

 at the end of thirty-four hours when observations were discon- 

 tinued. The spermatozoa of 58 in his own serum were wholly 

 immobilized at the end of five hours, while immobilization did 

 not occur in normal rabbit blood-serum until the end of twenty- 

 five hours. 



Male 32A1, substituted for the male (53B2) which developed 

 paralysis of the hind legs, was injected with his own spermatozoa 

 on the following dates: January 17th, 24th, 31st, and February 

 7th. His spermatozoa and blood-serum were tested February 



