248 



BESSIE NOTES 



centages of alcohol was so low (less than 25 per cent) that it soon 

 became evident that it would be impossible to continue the 

 lines indefinitely. Hence the line in 2 per cent alcohol was 

 discontinued and the progeny produced in 1 per cent alcohol 

 divided into two lots, one to be subjected to I per cent alcohol, the 

 other to | per cent. The organisms were reared for two weeks 

 over these percentages of alcohol; isolation of eggs for new genera- 

 tions were made whenever the age of the majority of adults 



TABLE 4 

 Diagrammatic scheme of alcohol experiment 



Feb. 1919-3ept. 1919. 

 Malted milk. 



1" xV.. 



2% ethyl alcohol. 



1 wk. 

 Malted milk 



1 wk. 



3,4" wks. 5" wk. 5,7" wks. 



Malted milk. 



2 wks. 



ethyl alcohol. 



2 wks. 

 ^ ethyl alcohol. 



2 wks. 



Malted milk. 

 2 wks. 



^a alcohol. 



2 wks. 

 i^fo alcohol. 



2 wks. 



9,10" wks. 11" wk. 



12,13" wks. 14" wk.15,16" wks. 17" wk. 18,19" wks. 20" wk 



Malted milk. 



2 wks. 

 5^ alcohol. 



2 wks. 

 i^o alcohol. 



2 wks. 



-^ Malted milk. 



2 wks. 

 -^ M alcohol. 



2 wks. 

 ■^ i% alcohol. 



2 wks. 



-^ Malted milk. 



2 wks. 

 ^ i^c alcohol. 



2 wks . 

 ^ alcohol. ■ 



2 wks. 

 Vfa alcohol. 



2 wks. 

 2°/? alcohol. 



2 wks. 



— ^ Malted milk. 



2 wks. 

 — > ifi alcohol. 



2 wks. 

 — > ^ alcohol. 



2 wks. 



21,22" wks. 23 "wk. 



25" wk. 26" wk. 



Malted milk. 



2 wks. 

 5^ alcohol. 



2 wks. 

 ^ alcohol. 



2 wks. 



-> Malted milk. 



1 generation. 

 -^Malted milk. 



1 generation. 



Malted milk. 



1 generation. 



-^ Malted milk. 



1 generation. 

 -^ Malted milk. 



1 generation. 

 -> Malted milk. 



1 generation. 



seemed to warrant. At the end of this time (five weeks) 100 

 individuals from each line were isolated and the egg production 

 and length of life determined. In the | per cent alcohol the 

 range of egg production was from 4 to 22, with an average of 

 14.25; the range of life from 3 to 7 days, with an average length 

 of life of 5.83 days in the | per cent alcohol the range in egg pro- 

 duction was from 1 to 10, with an average of 3.98; the range of 

 of life from 4 to 7 days, with an average of 6.09 days; in the 

 controls the egg production ranged from 14 to 30, with an average 

 of 14.10 eggs; the length of life from 3 to 7 days, with an average 



