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JOSEPH HALL BODINE 



when an animal is exposed to small doses of ether for such long 

 periods. The conclusion might reasonably have been dra^vn 

 from these experiments that no change in rate of CO2 output 

 takes place. 



Chloroform. The action of chloroform upon grasshoppers is 

 decidedly more marked and toxic than ether. The smallest 

 dose, 0.04 cc, for an hour produces irreversible effects. Res- 

 piratory movements with such a dose cease in a few minutes 

 (one to two). 



Fig. 3 Curves showing the effect of 2 drops — 0.08 cc. — chloroform" (curves 

 A, B, and C) on the CO2 production of grasshoppers. The point marked on the 

 abscissa indicates the beginning of exposure to chloroform. Previous to this the 

 normal production of CO2 was determined. The normal rate (which is taken as 

 100 per cent) corresponds to the production of a definite amount of CO2 in 11.7 

 minutes for curve A, in 12 minutes for curve B, and 5 minutes for curve C. Curves 

 A, B, and C irreversible. For further explanation see text. 



Figure 3 shows graphically the effects of chloroform on the 

 CO2 output. With small doses (one or two drops) a decrease 

 takes place, w^hich is followed by an increase; this in turn is 

 followed by a decrease. Experiments in which CO2 determina- 

 tions were made after cessation of respiratory movements gave 

 similar results, thus showing that the stopping of respiratory 

 movements was not accountable for the falling off in CO2 output. 



'When small doses of ether (4 drops) and of chloroform (2 drops) 

 are used together, the curve obtained is like that for chloroform 



