SKIN TRANSPLANTATION IN FROG TADPOLES 



359 



the amount of muscle tissue included was very small. Text 

 figure B illustrates the shape of such a graft and the place from 

 which it was taken. 



After the transplant was fitted to the wound the animal, 

 placed on a concave support, was lowered into a small battery 

 jar, containing enough 0.04 per cent chloretone solution to cover 

 the tail, ventral body region, and the mouth, while the wound 

 with its graft was not covered. The tadpoles were then kept 

 for two hours under these conditions, during which time the 

 transplant established union with the edges of the surrounding 





Text-figure A Diagram of the incision made in the integument around the 

 eye. A narrow band of skin was removed, leaving the conjunctiva and some or 

 the surrounding skin intact. The graft was then fitted to the incision. X 4. 



Text-figure B Diagram of the posterior tail region from which the tail -skin 

 grafts were taken. Dotted lines indicate the limits of such a graft. X 4. 



integument. It was necessary to replace the chloi.^one solution 

 by water whenever the respiratory rate became tco low. This 

 method of allowing the graft to unite with the surrounding nor- 

 mal integument, depending, as it did, on the coagulated serum 

 and blood to hold the transplant in place, has obvious advan- 

 tages over suturing or bandaging methods. At the end of two 

 hours, the animals were placed in fresh tap-water to recover from 

 the anaesthetic. Observations made daily thereafter consisted 

 of recording any noticeable changes of form, position, pigmenta- 

 tion, and proliferation of the transplant and making sketches or 

 photographs. For these purposes a glass cell (75 mm. long, 20 

 mm. wide, 25 mm. deep) containing water or chloretone solution 



