310 WHEELER. (Vo. IIf. 
first polar spindle remaining within the egg now forms another 
spindle directed like: the first. This is seen in\Pie, 14. The 
blade of the microtome has somewhat raised the loosely at- 
tached first polar globule, the protoplasm of which is seen to 
contain many of Blochmann’s corpuscles. The karyokinesis 
of the second spindle progresses in the same manner as the 
first and the second polar globule is given off, also surrounded 
by bacillar protoplasm. The portion of the nucleus which 
becomes the female pronucleus, is not seen in Fig. 15, where 
only the two polar globules are represented, as it appears in the 
next section. Figure 16 is froma surface view of the polar glob- 
ules. By comparison with Fig. 14 fg/ 1 it will be seen that they 
are lenticular. Here the female pronucleus appears as a more 
indistinct body (because out of focus) lying between the polar 
globules. The polar globules which I have been able to find in 
many eggs taken from the capsules while they were still vertical 
in the genital armature (about 6 to 12 hours after the begin- 
ning of oviposition) lie in the middle of the convex dorsal wall 
of the odthecal egg. They do not divide subsequently to form 
what Weismann (48) calls secondary polar globules, but soon 
disintegrate. In eggs about one day old their remains may be 
found as an amorphous granular mass, lying just beneath the 
chorion and entirely separated from the egg. 
The female pronucleus increases considerably in size before 
leaving the surface of the egg (Fig. 17 9 pz). This increase is 
gradual but constant as it makes its way through the dense 
yolk of the interior of the egg to the apex of the isosceles tri- 
angle of homogeneous yolk abutting on the back part of the 
granular ventral yolk. It is on this journey that the female 
pronucleus meets the male pronucleus formed from a spermato- 
zoon which has entered the egg by one of the funnel-shaped 
micropyles on the upper ventral face. 
Though I have succeeded in throwing a little more light on 
the copulation of the pronuclei than Blochmann, I cannot regard 
my observations as completely satisfactory. The process must 
be studied in Arthropod eggs with more evenly compact yolk 
than the eggs of Orthoptera, the numerous cracks and fissures 
in which render the observation of delicate internal processes 
exceedingly difficult if not impossible. Moreover, the copula- 
tion of the pronuclei in 2/a¢fa is hurried through very rapidly. 
