318 WHEELER. (Vou. IL. 
nected with intervitelline protoplasmic trabecule (Fig. 56 77). 
The karyoplasm of the nucleus is coarsely granular. In the 
nucleus figured only two of the vacuolated masses of chromatin 
(or nucleoli, as most writers call them) are present. The larger 
contains six vacuoles, one of which incloses a bar-shaped mass 
of chromatin (Fig. 56 7/7). Inthe cavity of the nucleus a number 
of more or less oval hyaline masses are seen. They are doubt- 
less the equivalents of the “ maturation spheres,’ noted above 
in Blatta. Stuhlmann (45) has found these same spheres in 
the degenerating germinal vesicle of ZLzza, a Chrysomelid allied 
to Doryphora. 
The next stage found in the decomposition of the germinal 
vesicle is represented in Fig. 57. The karyoplasm has become 
confluent with the intervitelline protoplasm, and only the chro- 
matin portion marks the spot where the nucleus reached the sur- 
face of the egg. The larger yolk spheres, formerly present even 
in the surface protoplasm, have passed inwards, and only the 
smaller spheres still remain in what is to become the blastema. 
Soon these, too, retire further into the egg, and the surface pro- 
toplasm is marked off from the vitelliferous portion. The re- 
mains of the nucleoli are worthy of attention. The vacuoles have 
disappeared, and the glistening chromatin has grown denser, 
and stains very deeply. In the case figured, one or two nucleoli 
have evidently broken into the nine fragments of different sizes 
and shapes. The larger and more peripheral mass (Fig. 57 72) 
is surrounded bya pale aureole. Its size, position, and the 
clear protoplasm surrounding it, seem to point it out as the im- 
portant and permanent mass of chromatin soon to be converted 
into the first polar spindle. The remaining eight fragments 
seem to be leaving the surface and passing into the yolk, where 
they probably disappear, as no traces of them can be found in 
succeeding stages. 
In Fig. 58, which represents the next stage in the nuclear 
metamorphosis, we find that the mass # of Fig. 57, which con- 
tained five spherical masses of dense chromatin, has become a 
perfect oval nucleus in the resting stage. The chromatin has 
again passed into the filamentous state. The surface layer 
of protoplasm is clearly developed and has secreted the vitelline 
membrane (Fig. 58 v). The chorion, too (Fig. 58 ch), has ap- 
peared. The follicular epithelium which secreted it is omitted 
in this and the next figures. 
