No; 2:'] BLATTA AND DORYPHORA. 319 
The resting nucleus just described soon begins to divide. Its 
small size prevents an accurate understanding of the peculiari- 
ties of its mode of division. Enough can be gleaned from Fig. 
59, however, to show that the karyokinesis is not typical like 
that described by Flemming (12, 13) and Rabl (41) for the 
epithelial nuclei of Sa/amandra. No loops seem to be present 
in the metakinetic stage, but the chromatin is arranged in mo- 
niliform strings, each of which seems to be applied full length 
to one of the achromatic fibres of the spindle. I have not seen 
all the stages in the metakinetic process. 
In the next stage observed (Fig. 60) the two masses of chro. 
matin resulting from metakinesis have reached the poles of the 
spindle, where the fibres before apparent have become invisible. 
In the equatorial plane, however, the achromatic filaments are 
very distinct, being noticeably thickened. This thickening of 
the spindle fibres to form the dividing plate (4) between the two 
cells is of universal occurrence in plants, as may be seen from an 
examination of Strasburger’s (44) delicate figures. It is, how- 
ever, not infrequent in Arthropods. Carnoy (g) figures many 
instances in his “ Cytodiérese.” 
Unfortunately I have been unable, through lack of material, 
to trace the changes of the nucleus immediately following those 
just described. The ovum is deposited by the female Doryphora 
with its nucleus is the stage represented in Fig. 60 (compare 
Blatta). The outer mass of chromatin (/!) must be regarded 
as the first polar globule. Probably the process of forming the 
second polar globe is essentially the same as in A/atta. 
Though incomplete, my observations prove, I think, that polar 
globules are also formed in the Coleoptera, thus adding another 
order of insects to the three in which these interesting bodies 
were found by Blochmann (5). 
As in 4/atta, the nuclei represented in Figs. 57 to 60 are very 
small and difficult to find in the enormously larger eggs. 
Long before oviposition the eggs of Doryphora have acquired 
the dull orange color which makes them so conspicuous on the 
under surfaces of the leaves to which they are glued by the 
females. The coloring-matter is seated in the yolk bodies, and 
ultimately disappears in eggs preserved in 70 per cent alcohol. 
The eggs are most deeply colored in living specimens in the 
earlier stages, and a gradual fading of the color accompanies the 
