FEEDING PARAMECIUM KNOWN BACTERIA 141 



Agar colonies: amoeboid, spreading, smooth, glistening, growth rapid, 

 non-chromogenic , 



Staining reactions : Gram positive. 



Gelatin: rapidlj^ liquefied, saccvilate, growth best at top, plumose to 

 villous. 



Litmus milk: slightly decolorized at bottom of tube after four days, 

 slowly becoming entirely decolorized, completely peptonized after 

 thirty days. 



Bouillon: pellicle formed. 



Dextrose bouillon : no acid, no gas, membrane formed. 



Lactose bouillon: no acid, no gas, membrane formed. 



Saccharose bouillon: no acid, no gas, membrane formed. 



Mannite bouillon: no acid, no gas, flaky sediment at bottom. 



Organism J . Belongs to class VIII, B. subflavus type of Chester. 



Isolated from a one- to three-day hay infusion. 



Morphology: short rod, almost spherical, markedly motile. 



Agar colonies: compact, slight tendency toward radiation, slightly 

 chromogenic, yellow. 



Staining reactions: capsulated. Gram positive. 



Gelatin: not liquefied, growth spreading over top, growth best at top, 

 filiform, beaded along edge. 



Bouillon: clear, membrane at top. 



Dextrose bouillon: no acid, no gas, membrane present, flocculent 

 sediment, medium later becomes cloudy. 



Lactose bouillon: no acid, no gas, membrane present, flocculent 

 sediment, medium later becomes cloudy. 



Saccharose bouillon: no acid, no gas, membrane present, flocculent 

 sediment, medium later becomes cloudy. 



Mannite bouillon: no acid, no gas, membrane present, flocculent 

 sediment, medium later becomes cloudy. 



Organism K. Class IV of Chester. A micrococcus. Isolated from 

 a one- to three-day hay infusion. 



Morphology: small spheres, may occur in chains, cells vacuolated, 

 non-motile. 



Agar colonies : grow slowly, moist, edge sharply defined, non-chromo- 

 genic. 



Staining reactions: Gram positive. 



Gelatin: no liquefaction after eleven days, growth uniform and 

 filiform. 



Litmus milk: becomes slowly alkaline, shghtly decolorized, slightly 

 peptonized, later is completely decolorized and peptonized. 



Bouillon: clear. 



Dextrose bouillon: no acid, no gas, cloudy. 



Lactose bouillon : no acid, no gas, cloudy with sediment. 



Saccharose bouillon: no acid, no gas, cloudy, heavy sediment. 



Mannite bouillon: no acid, no gas, cloudy with small colonies 

 scattered throughout the medium, cloudiness slowly disappears to be 

 replaced by sediment. 



