142 RUTH L. PHILLIPS 



Organism L. Class XV, group II B, the subtilis group of Chester. 

 Isolated from a nine- to fourteen-day hay infusion. 



Morphology: long rods, sometimes single, sometimes in chains of 

 two, endospores central, motile. 



Agar colonies: round, moist, glistening, edge sharply defined, slightly 

 indented, granular, non-chromogenic, medium strongly discolored, 

 brown. 



Staining reactions : Gram positive. 



Gelatin: liquefied rapidly, growth sacculate, filiform. 



Litmus milk: initial coagulation followed by decolorization and 

 peptonization. 



Bouillon: flocculent cloudiness. 



Dextrose bouillon: no acid, no gas, pellicle at top. 



Lactose bouillon: no acid, no gas, pellicle at top. 



Saccharose bouillon: no acid, no gas, pellicle at top. 



Mannite bouillon: no acid, no gas, growth cloudy, especially below 

 membrane. 



Organism A'. Bacterium class VI, group II, of Chester, possibly 

 B. Luteum. Isolated from a nine- to fourteen-day hay infusion. 



Morphology: small rods, non-motile. 



Agar colonies: round, convex, moist, opaque, edge slightly irregular, 

 chromogenic, deep yellow. 



Staining reactions: dark staining granules at one end, Gram posi- 

 tive. 



Gelatin: no liquefaction at first, liciuefied after two months, growth 

 best at top, yellow, filiform. 



Litmus milk: slightly alkahne at first, slowly decolorizing and 

 peptonizing. 



Bouillon: yellow pellicle at top, medium fairly clear with flocculent 

 sediment. 



Dextrose bouillon: no acid, no gas, slight pellicle. 



I actose bouillon: no acid, no gas. 



Saccharose bouillon: no acid, no gas. 



Mannite bouillon: no acid, no gas. 

 Organism B' . Class VII, group II, of Chester, possibly B. latericium 

 or B. havaniensis. Isolated from an old culture in which Paramecium 

 were not at the time thriving. 



Morphology: short rod, non-motile. 



Agar colonies: very slow growing, round, moist, raised, opaque at 

 center, edge indefinite, chromogenic, brick red. 



Staining reactions: Gram negative. 



Gelatin: not Hquefied, growth best at top, spreading, beaded along 

 line of stab. 



Litmus milk: neutral at first, followed by decolorization and alka- 

 linity. 



Bouillon: red sediment, red growth at top. 



Dextrose bouillon: no acid, no gas, flocculent sediment, red growth at 

 top. 



