452 



DWIGHT E. MINNICH 



The 5th, 6th, and 7th days, no trials were made with saccharose 

 since the animal responded to water. During the 7th day 

 the animal became so weak that further experimentation was 

 impossible. Accordingly, trials were suspended, water was 

 administered, and the butterfly was allowed to recuperate. 



On the following morning, viz., the morning of the 8th day, 

 the butterfly had entirely recovered, its responsiveness to water 

 had disappeared, and trials with saccharose were resumed. As 

 the period of water diet continued, the threshold of response to 

 saccharose gradually fell from M/400 on the 8th day to M/3200 

 on the 11th day. At the close of the 11th day the animal again 

 evinced signs of weakness, and it was necessary to change the 

 diet to IM saccharose. 



Absence from the laboratory prevented any trials on the 12th 

 day. When experimentation was renewed on the morning of 

 the 13th day, however, the butterfly had completely recovered its 

 normal vigor. But the threshold of response had risen from 

 M/3200 to M/10, and at this level it steadily remained through- 

 out the entire period of saccharose diet, viz., the 13th, 14th, and 

 15th days. 



Following the first period of saccharose diet came the second 

 period of total inanition, which lasted from the 16th to the 20th 

 days, inclusive. During the first two days of this period, the 



