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Nachdruck verboten. 



A new Staining 3Iethod for bile Canaliculae. 



By Dr. B. Morgan Vance, Eesident Pathologist. 



The Pathological Department, Bellevue Hospital, !New York City. 

 Dr. Charles N orris, Direktor. 



The method now most employed, to demonstrate the bile canali- 

 culae in sections of liver, is the one described by Eppinger (Ziegler's 

 Beiträge, Band 31). While this technique effectually stains the 

 canaliculi, it is a complicated process, and one that requires a long 

 time to complete satisfactorily. The method given below is a simple 

 and short technique, while at the same time it consistently and clearly 

 demonstrates the bile canaliculae. 



It is as follows: 



1. Fix preferably in either of the following mixtures: 



a) Equal parts of Zenker's fluid minus the acetic acid and 10% 

 aqueous solution of formalin, 



b) Equal parts of 10% formalin and 5% Mercuric Bichloride 

 aqueous solution. 



2. Harden. 



3. Embed in celloidin. 



4. Section, and place sections in a dilute solution of iodine in 96% 

 alcohol for five to fifteen minutes. 



5. Wash in successive changes of 95% alcohol to remove iodine. 



6. Stain in Phospho-tungstic Acid Hemotoxylin (Mallory, Journal 

 of Experimental Medicine, No. 5, 1900) for twelve to twenty-four 

 hours. 



7. Transfer directly to 95% alcohol and wash. 



8. Clear in Xylol-carbol or oil of origanum. 



9. Balsam. 



Formalin fixed tissue can be used if the celloidin sections are 

 placed in a saturated solution of Mercury Bichloride for fifteen to 

 thirty minutes; then transferred to the alcoholic solution of iodine, 

 and the process completed as above. Paraffin sections do not stain 



