86 



this time. These then represent about as normal a group of indi- 

 viduals as we are likeh^ to find. 



Subjects Nos. 1 and 2, September 1910, Filipinos, were injected 

 with neutral red 1 : 20,000. The staining was so deep that a count 

 or estimate was impossible in each case. 



Subject Nr. 3, October 14, 1910, Filipino, adult about 34 years 

 old and weighing about 130 lbs. wasinjected with a smaller amount of 

 neutral red than 1 : 20,000. Afairlvgood stain was obtained and 353,765 

 islands were estimated. As there was not sufficient blotting paper 

 at the prison hospital to extract all the water from the pancreas and 

 as the balances were not delicate, these results, beyond indicating 

 the presence of a great number of islands in the human pancreas, are 

 not of distinct value. 



Subject No. 4, February 23, 1912, Filipino, adult, about 24 j-ears, 

 weight about 140 lbs., healthy and very robusts was injected with 

 neutral red 1 : 40,000. Weight of pancreas 87 grams ; estimated 

 total number of islands, 1,760,000, or 20 — 23 islets per milligram of 

 fresh pancreas. 



Subject No. 5, December 2, 1911; Chinese baby of six months, 

 female, weight 5,5 kilos; death caused by violence; pancreas injected 

 with neutral red 1 : 50,000 soon after death ; weight of pancreas 

 5,582 grams; estimated total number of islands 120,323; number of 

 islands per milligram of fresh pancreas 21.55 (exclusive of a vast 

 number of 1 to 6 celled islands which are not distinguishable under 

 the low power lens used for counting). 108 mg. gave 2328 islands, 

 whose distribution Avas not studied. It was thought worth while to 

 make a high power study of this pancreas. This was why only 108 mg. 

 were counted ; see below. 



Subject No. 6, January 16, 1912. Young Filipina mother 22 

 years old, about 105 lbs., who died suddenly in second pregnancy 

 (4 weeks ectopic). The pancreas was injected 2^/2 hours after death 

 with 1 : 30,000 solution of janus green in 0,9% salt solution. 92 mg. 

 from various parts of pancreas gave 1707 islands. Further counts 

 were not reliable; estimated total number of islands 1,534,085; 

 weight of pancreas 82.7 grammes; number of islands of Langerhans 

 per niilHgram of fresh pancreas, 18.55. 



Subject No. 7, Feb. 16, 1912, Filipino, age about 45, weight 

 about 125 lbs., injected with 1 : 30,000 janus green in Einger's 



