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though up to the present time reproduction by budding, the only funda- 

 mental characterestic of this subdivision of the Tunicata, has not been 

 proven to occur in any of them. 



A recently discovered but still undescribed species (Goodsiria 

 dura, I shall name it) from Santa Barbara, coast of California, enables 

 me not only to convert the belief that these Ascidians reproduce by 

 budding into actual knowledge, but also to give a full account of the 

 development of the blastozooids. 



In this preliminary communication 1 propose to give in the barest 

 statement — nothing more — the most important results of my study 

 of the budding in this new species, and also in the genus Perophora. 



A detailed and illustrated account of my work, with some general 

 considerations will appear later, as time permits the preparation of it. 



In the development of the bud in Goodsiria, the following are 

 the facts of chief importance: 



1) The budding is "pallial", to use Giaed's term ; it being, then, 

 the same as in the Botryllidae. 



The bud arises as an evagination of the wall of the peribranchial 

 sac, its position being, in all cases yet observed, far forward on the 

 mother ascidiozooid. 



2) Buds appear never to arise from fully adult ascidiozooids. The 

 oldest mother ascidiozooid on which I have found the anläge of a 

 bud, is at a stage of development in which the branchial siphon is 

 about to break through, and only a comparatively small number of 

 stigmata are formed in the branchial sac. 



3) The bud becomes entirely severed from its parent 

 at a very early stage in its development; i. e. before there is any 

 trace of differentiation of organs. At this time "endoderm" and ecto- 

 derm are spherical vesicles entirely closed at all points, the 

 former situated concentrically within the latter, but separated from it 

 by a considerable space. This space contains many blood-cells derived 

 directly from the parent ascidiozooid. 



4) There exist in the common testicular mass great numbers of 

 much branched, anastomosing vessels which terminate in large am- 

 pullae. These vessels contain no "cloison", or partition such as exists 

 in the stolon prolifer of some other Ascidians, and which plays so 

 important a role in the production of the blastozooids. 



I have no evidence that buds are ever produced in connection 

 with these vessels, and it is quite certain that they are not true 

 stolons any more than are the similar vessels in Botryllus and some 

 other genera. 



