82 
whatever; pieces from this material being afterwards treated witk 
water, 70°/, alcohol and iodine alcohol to remove any remaining traces 
of sublimate. 
As soon as the iodine alcohol ceased discoloring, the pieces were 
washed for some time in 90°/, alcohol, then transferred to absolute 
alcohol, afterwards toluol paraffin and at last pure paraffin, sections 
being afterwards cut in the usual way by microtome. 
Different staining methods were tried, as, for instance, haemato- 
xylin-eosin, carmine and picro-carmine, but I obtained the best re- 
sults with the Enriica-Bionpr staining fluid. 
Pieces of fresh tissue were, to differentiate the nerves, treated 
with methylin-blue according to EHRLICH-DOGIEL and the color fixed 
with BETHE’s method. 
If sections are cut through the gland and viewed by low magni- 
fication, it will be found that the gland consists of a number of lobes 
kept together by connective tissue and the whole enclosed in a cap- 
sule consisting of two strata, the inner being entirely composed of 
connective tissue, the outer of a reticular fabric, this being partly 
connective tissue, partly elastic fibres. From the inner of these layers 
the septa that separate the different lobes are derived, as well as all 
connective tissue in the interior of the lobes. The outer layer is 
transversed by numerous bloodvessels, as well as nerves. The blood- 
vessels that supply the interior of the gland run between two lobes 
and the membrane which covers the gland (Fig. 1, B. V.); from 
these vessels the interlobular ones arise. Each lobe is divided by a 
number of radial septa into sectors, which are composed of a mass 
of secreting tubes, separated from each other by connective tissue. 
This tissue carries the blood-capillaries and in the thicker septa a 
number of capillaries are seen perforating them in all directions 
(Fig. 2, B. V.). The secreting tubes run in all directions and 
are generally convoluted and of considerable length, but sometimes 
straight, radial tubes may be found that run from the central duct 
to the periphery of the lobe. If a longitudinal section of one of the 
secreting tubes is observed, it will be found that the cells in different 
parts of this tube are in different secretatory conditions. In the un- 
ripe cells the protoplasmic contents are hardly stained by the Bronpr 
fluid and the granula are hardly visible, being very small and the 
whole cell is surrounded by a strong ectoplasmic membrane, but follow- 
ing the cells along the tube, the contents will be found to be darker 
and of distinct granular appearance, the interior cell-membrane at the 
same time getting thinner and less distinct, until, ultimately, in the 
