328. 



MATSUZIRO TAKENOUCHI 



•' , Rabbit, group B, no. 1 



, Date of injection Amount of testis emulsion injected 



'_ July 18, 1918 4 testes from two albino rats 



' '' July 23, 1919 6 testes from three albino rats 



■ August 1, 1918 6 testes from three albino rats 



August 23, 1918 8 testes from four albino rats 



September 1, 1918 The blood was taken 



' The emulsion of testes was prepared by grinding the testes in a 

 mortar with physiological saline solution. This was strained 

 through cotton gauze to free it from the particles of connective 

 tissue. 



The emulsion was prepared each time just before the injec- 

 tion, so that we did not need to make use of any disinfectant 

 to. mix with the emulsion for conservation. 



, The autopsy of the rabbit showed no pathological changes. 

 The serum was separated carefully and preserved with carbolic 

 acid (0.5 per cent) and used for the tests in vitro as well as 

 in Vivo. 



a. Test of the antitestis serum in vitro 



The precipitin reaction of the antitestis serum with extracts 

 of several rat organs was tried with the results given in table 6. 

 The technique used in the preparation of the extracts and the 

 ihethod for the precipitin test were those used in the test of the 

 antithymus serum, and therefore the description may be omitted 

 here. 



• ' TABLE 6 



Pi'ecijritin reaction of the antitestis serum with the extracts of different organs of 

 the albino rat 



Extract 



Antitestis serum, in drops 



Result after 6 to 7 hours in ice-box 



<1), Kidney.....: 



.'.. (,2) Teptes 



'' (3) Thymus 



(4) Spleen 



! (6) Brain,..* 



1.0 



1 



+ + 



1.0 



2 



+ + 

 + 



1.0 

 3 



+ + + 

 + + 



1.0 



4 



+ 



+ + + 



+ + + 



+ + 



1.0 

 5 



+ + 

 + + + 

 +++ 

 ++ + 



1.0 



