220 



twelve spherical granules which stain deeply with iron hematoxylin 

 (Fig. 9), but remain transparent and highly refractive in Congo — 

 Ehrlich's hematoxylin preparation (Fig. 10). Rarely a second nucle- 

 olus of smaller size may be seen (Fig. 6, b, nll 2 ). 



The karyoplasm immediately about the nucleolus often stains less 

 deeply than in its more peripheral parts. This may be attributed to 

 its being greatly vacuolated in that region. This lightly staining region 

 varies much in size and in the definiteness of its outline, the maximum 

 in this respect being shown in Fig. 8 (wZ 2 ), which is an unusual con- 

 dition and may be due to faulty preservation. In this cell the chro- 

 matin net seen faintly may be followed from the lighter to the darker 

 area (at the right in the Figure) thus showing the continuity of the 



Fig. 8. Fig. 9. 



nl 2 nil. nlc £ nl. nl 2 



I— nil. 



cyfpl I Cyi ' pl 



£ b 



Wt- ax.cyl. 



Fig. 8 Giant cell from the dorsal fissure of the cord, showing an abnormally 

 large nucleus (nl.) with the vacuolated area (nl%.) surrounding the nucleolus (nil.), ax. cyl. 

 axis cylinder; cyt. cytoplasm» Iron haematoxylin after Flemming. Camera drawing; 

 obj. '/is °*' imm., oc. 4. 



Fig. 9. Giant cell. The nucleolus (nil.) shows Eark granules. Abbreviations as 

 in Fig. 8. Ehblich's haematoxylin, congo red. Camera drawing ; obj. 7 a, oc. 2, details 

 with 1 / 12 oil imm. 



karyoplasm in the two parts. Usually this lighter area is relatively 

 small (Fig. 9, nl 2 ) and its limits indistinct, the denser karyoplasm 

 becoming gradually lighter toward the nucleolus (Fig. 10). Sometimes 



