Fig. 1. Fig. 2. 
Figs. 1 and 2. Semidiagrammatie figure of the reticulum of the Zona glomerulosa 
of the dog’s adrenal. Fig, 1 prepared by SPALTEHOLZ’ method. Fig. 2 by MaAur’s 
method. Magnified about 120 diameters. 
C capsule. Z. @. Zona glomerulosa. Z, F. Zona fasciculata. c small process 
limiting cell column of Zona glomerulosa. S large septum from the capsule to the Zona 
reticularis. 5 septum cell spaces of the Zona glomerulosa. 
different methods of manipulation. For example, the sections in MALL’s 
method are thick and one is thus able to obtain a suggestion of the 
third dimension so that the reticulum fibres can be traced throughout 
their entire course, although the violent shaking which is necessary 
to remove all of the cellular debris is liable to displace many of them. 
The relations of the fibrils; on the other hand, in the SPALTEHOLZ 
preparations’ are admirably preserved inasmuch as they are fixed in 
situ and can be compared with stained controls but owing to the fact 
that the sections are so thin and the fibrils cut in such small seg- 
ments it is impossible to trace them for any distance. The larger 
septa in the SPALTEHOLZ sections often appear black and homogeneous, 
while by Marr’s method the individual fibrils comprising them are 
clearly stained. I have found, however, that the fibrillar structure of 
the septa in sections prepared by SPALTEHOLZ’ method can often be 
seen when they are studied with an immersion lens. Accordingly, I 
believe that these two methods are mutually explanatory and in the 
study of the reticulated tissues, in general, should always to be used 
together inasmuch as neither method, in itself, is sufficient to explain 
all of the relations of the fibrils in an organ. 
