PHYSIOLOGY OF THE INVERTEBRATA. 155 



When the blood is treated with alcohol and potassium 

 hydroxide and filtered, a vellow-coloured solution is obtained 

 " free from bands, but on adding ammonium sulphide a band 

 appears covering 1) " (Fig. 30, d). " On treating aqueous 

 solutions with acetic acid the bands faded away, and the 

 colour of the solution changed to a brownish colour (gaslight)." 



Mac^Munn tried the action of alcohol acidulated with 

 sulphuric acid on chlorocruorin, and obtained a greenish 

 solution, which showed a faint shading in the green, too 

 indistinct to map. 



" Hence none of the decomposition products of haemoglobin 

 or ha^matin could be obtained, the pigment, as Prof. Lan- 

 kester had already shown, being destroyed by the reagents 

 required to produce acid hsematin and hferaatoporphyrin. 

 The blood of the pseudo-ha3mal system of Serpula contortu- 

 2)licata presents some resemblance to that of Sahella, There are 

 slight differences in the blood spectra of some specimens, which 

 doubtless are due to the pigment being present in different 

 states of oxidation, and on comparing some of these spectra 

 with those of the histoha?matins and with the decomposition 

 products of haemoglobin, a striking likeness is apparent." 



" On putting a Scqyi'hc into the compressorium, and bring- 

 ing gentle pressure to bear on the upper surface of the 

 animal, and examining with the microspectroscope, using a 

 good achromatic substago condenser, a series of spectra are 

 obtained when the various parts of the animal are moved 

 under the objective ; what these parts are is seen by looking 

 down the left-hand tube of the microscope. In this way we 

 can differentiate the blood-vessels, intestine, gills, opercu- 

 lum, and other parts, and study the spectrum of each." 



With the pseudo- hajmal system of Serpulo^ ]\racMunn 

 obtained a spectrum represented in Fig. 30, 6'. The band 

 before D is like that of Lankester's chlorocruorin, but the 

 first after D and also the second are different. 



An aqueous solution of the blood from the pseudo-hajmal 

 system is yellow by daylight, reddish- yellow by gaslight, and 



