PHYSIOLOGY OF THE INVERTEBRATA. 225 



says : *' No chlorocruorin, no liaemoglobin, no chlorophyll was 

 present, and no discernible lipochrome. The solid pigment 

 became reddish-brown with sulphuric acid, and red-brown 

 with nitric acid." MacMunn simply names this colouring 

 matter phyllodoce-green, as it is impossible to refer it to any 

 class of animal pigments. 



In Pontohddla, a pigment, bearing a remote resemblance 

 to chlorophyll, has been extracted from the integument 

 where it occurs in large pigmented cells of a green colour. 

 This worm, belonging to the Hirudinca, although it lives 

 on fish-blood, is capable of manufacturing from its food a 

 colouring-matter allied to chlorophyll. This pigment is 

 soluble in alcohol and ether. 



Mr. r. E. Beddard, F.R.S.E.,* has examined the glandular 

 cells in the integument of JEolosoma tenehrancm and other 

 species of this genus. In the sjDecies mentioned these cells 

 are nucleated, and in the centre is a large globule of oily 

 appearance impregnated with a green colouring-matter. 

 Yejdovsky f states that this globule is stained black with 

 osmic acid ; but Beddard found that this acid stained the 

 globule a brown colour. Various reactions given in Bed- 

 dard's paper show that the green colouring-matter of this 

 Avorm is not chlorophyll. In its behaviour with acids and 

 alkalies it resembles certain pigments described by Prof. 

 Moseley,:^ Dr. MacMunn, and others ; and there is little 

 doubt that it has a respiratory function. This pigment is 

 different from bonellein and chlorocruorin, two pigments 

 present in certain Annelids. The blood of JEolosoma tene- 

 hrarum is colourless, and there are no special respiratory 

 pigments in other parts of the body ; therefore, as Beddard 

 justly remarks, " the pigment of the integumental glands 

 may perform the function of respiration." 



* Proceedhif/s of the Zoological Society, 1889, p. 51; and Annals and 

 Marjazine of Natural History, 1889, p. 262. 

 t Thierische Orrjanismen dar Brunnenwdsser von Prag, p. 61 [1882]. 

 % Quart. Journ. Micros. Science, vol. 17. 



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