m 



gave excellent demonstrations of zymogen granules when treated with 

 iron-hsematoxylin, while their nuclei and protoplasm remained unaltered. 

 The connective tissue never showed the hyaline change. All these 

 trout stomachs contained food, generally the remains of small crusta- 

 ceans or insects, which they were actively digesting, and were there- 

 fore precisely in the condition where one would have expected post- 

 mortem digestion to be specially rapid. The salmon stomachs I have 

 described as showing this "catarrhal" change did not contain food and 

 were therefore not active, and it seems therefore reasonable to con- 

 clude that the catarrhal change was not due to this cause. The 

 stomach of the trout may quite fairly be compared with that of the 

 salmon, as it is merely a miniature copy of it. The main points of 

 difference between the two are that in the trout the glands are 

 shorter, the stratum compactum is very much thinner, the connective 

 tissue less in amount, and the muscular coats, of course, not so 

 strongly developed. The minute structure of the difierent elements of 

 the organ is precisely similar in the two fish. 



Moreover, in some stomachs from the salmon, notably in that 

 from No. 72, which was caught at sea, we had an opportunity of 

 seeing the change which really takes place post-mortem, as this salmon 

 had been out of the water 36 hours or more before it reached us. 

 In this case the connective tissue has the usual appearance, without 

 the hyaline change, the zymin-secreting cells are well preserved and 

 show no chromatolysis of their nuclei, and it is only the superficial 

 epithelium which is altered. In these cells the nuclei are shrunken, 

 the cell-bodies are swollen and agglutinated, so that their outline has 

 become less distinct, and they look much like the cells of mucous 

 glands after treatment with water. But the cells retain their arrange- 

 ment, and are not cast off as in the fish from the rivers. 



Stomachs of Kelts. 

 It was exceedingly interesting to find that these had regained the 

 normal appearance. The fish did not reach the laboratory till many 

 hours after death, so that there was a certain amount of the post- 

 mortem change in the superficial epithelium noted above. The zymin- 

 secreting epithelium, however, and the rest of the mucous membrane 

 were quite normal in every way, and the number of zymogen granules 

 in the cells was very large. 



The Pyloric Appendages and Intestine. 

 The appendages in the salmon are very numerous, and some doubt 

 has always prevailed as to their real significance, whether they are 



