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at the cut-surface of the fourth leg. The new part began to grow out 
soon after the operation and continued to enlarge for several weeks, 
when growth seemed to have ceased. No change of exoskeleton had 
taken place during this time and although the crayfish was kept alive 
for several months longer, the new part did not increase in size. In 
another case, the third walking leg had been cut off at the outer part 
of the fourth segment. For several months no regeneration could be 
seen at the cut-surface but at the end of this time the animal moulted 
when a new, clawed, distal part appeared as shown in Fig. 16. ‘This 
new part must have begun to develop inside of the last segment and when 
set free at the moulting period grew much larger. Another leg of the 
same animal that had been cut off at the same time did not produce 
a new distal end. A further case is shown in Fig. 17 in which a leg 
had been cut off at the joint between the second and third segments. 
After several months a small protuberance could be seen in the middle 
of the cut-surface, and when the segment was split open a new distal 
end appeared just beneath the protuberance and extending into it. 
During October of the current year (1901) I again collected a few 
crayfishes and repeated the last experiment. In one case in which one 
of the first pair of legs or chelae had been cut off through the middle 
of the fifth segment the stump was not thrown off. About forty-eight 
hours later the crayfish moulted and I observed a new, very small 
lump of new tissue projecting from the middle of the cut-end of the 
fifth segment. During the following two or three weeks the new part 
increased in size and in length. It was obviously destined to replace 
the missing part of the leg. 
In another crayfish operated upon at the same time the second 
walking leg was cut off at the base of the the fourth segment — the 
muscles inside of the breaking-joint having been previously cut. The 
stump remained attached and regenerated a new distal end from the 
cut-surface. 
These five cases show that the crayfish has the same power as 
the hermit-crab and can regenerate the distal portions of it legs when 
they are cut off outside of the breaking-joint. It has also been stated 
above that the crayfish possesses the power of regenerating a new leg 
when the old one has been cut off inside of the breaking-joint. I have 
shown that the hermit-crab has this same power and PRZIBRAM found 
later (1900) that in other decoped crustacea the same process takes 
place. These results show that there can be no necessary connection 
between the process of autotomy and the power of regeneration, or 
at least that the power of regeneration is not confined to the regions 
