204 STEIL: VEGETATIVE REPRODUCTION IN POLYPODIUM IRIOIDES 
and in respect to length resembled root hairs. None of the 
sporophytic outgrowths were connected with the vein of the leaf 
of the sporophyte. 
By March 4, 1919, the parent leaf had increased little in size. 
At this time it was observed that a leaf (m) of one of the complete 
sporophytes (>) had regenerated and produced in turn three out- 
growths (Fic. 1, 0, , and p), which later developed into leaves. 
The two complete sporophytes were detached and planted on 
Sphagnum. From qne of the sporophytes (b) a leaf (nm) was 
removed and was likewise planted on Sphagnum. The two sporo- 
phytes lived only a short time. From the detached leaf () it 
was discovered, May 12, 1919, that four outgrowths had been 
produced. Three of these were 
gametophytic, and the other sporo- 
phytic. A portion of the leaf and 
two of the young prothallia (m and 
n) are represented in Fic.2. One 
of the outgrowths produced a large 
cell (a), probably apical in nature: 
The three gametophytic or pro- 
thallial growths developed from the 
leaf of the sporophyte are apo- 
G Aposporously produced 
prothallia from a leaf of Polypodium n i 
SE rounding the gametophytic devel- 
opments are somewhat intermedi- 
ate between the two generations. The parent leaf soon showed 
signs of exhaustion and June 15, 1919, was partially dead. Later 
all of the outgrowths of the leaf died. 
Another sporophyte in the culture produced from each of the 
leaves a normal leaf. Roots were not developed. Since the 
sporophyte bore three leaves, one of the regenerating leaves was a 
secondary one. 
The remaining sporophyte bore four leaves, each of which 
developed one or more outgrowths. In one instance a leaf pro- 
duced a cylindrical structure distinctly gametophytic in its cellular 
nature. It produced numerous rhizoids at its point of origin. 
This gametophytic outgrowth was also of aposporous origin. A 
normal leaf was formed by another of the four leaves. At the 
sporous. The cells of the leaf sur- + 
a 
