STEIL: VEGETATIVE REPRODUCTION IN POLYPODIUM IRIOIDES 205 
base of the third leaf two small cellular masses appeared, probably 
sporophytic in nature. The fourth leaf produced a similar cellular 
mass, represented in section by 
: FR 
Fic. 3. In no case were these 
cellular masses connected with 
the vascular system of the leaf. 
It is of interest to note in this 
case that all of the leaves of 
the sporophyte produced out- 
growths. | 
The cultural conditions under 
which regeneration from the 
sporophyte and apospory occur in Polypodium trioides are not 
known to the writer. The phenomena appeared in an old culture 
in which the conditions may have been unfavorable for the normal 
development of the sporophyte. It may be added, however, that 
a fresh supply of a nutrient solution was on several occasions 
applied to the culture. 
UNIVERSITY OF WISCONSIN 
Fic. 3. Asection through a cellular 
mass produced from the leaf of Polypo- 
dium irioides, x 200. 
