272 STEIL: POLYPODIUM IRIOIDES 
growths were later produced. For a description of these the 
reader is referred to another paper.* 
The figures made as a result of this investigation were drawn 
with the aid of a camera lucida from living’ material. Some of 
the prothallia with the abnormal archegonia were fixed in Flem- 
ming’s medium fluid, imbedded in paraffin, sectioned and finally 
stained with safranin and light green. 
ANTHERIDIA 
In the autumn of 1918, when the first sporophytes were ob- - 
served to produce secondary sporophytes, the antheridia and | 
archegonia to be described were discovered. 
The majority of the antheridia appeared normal at this time, 
producing actively motile antherozoids. When these sex-organs 
were mature, the sterile cells contained 
only a few chloroplasts. A large number 
of abnormal antheridia developed among 
the normal ones. The lid cell and the two 
ring cells of the abnormal type of antheri- 
dium contained usually a large number of 
chloroplasts. Such antheridia were prim- 
arily vegetative in character and only 
occasionally produced mature antherozoids. 
These antheridia were not confined to the 
older prothallia, but were most frequently 
found on the younger filaments or second- 
- A portion of a ary prothallia, which (as has already been 
P rothallium of Polypodium tated) were commonly developed in the 
ment was produced. An Culture after some of the larger prothallia 
antheridium at the end of were removed. 
the filament developed a 
prothallium. X 20 
From the sterile cells of these antheri- 
dia prothallial filaments or secondary an- 
theridia were frequently produced. The most common cases 
of regeneration were observed in connection with the lid cell. 
Such filaments always consisted of a single row of cells. Early 
stages in the development of the filaments are represented in 
Fics. 1 and 2 of PLaTE 4. These filaments were observed to 
*Steil,W.N. Vegetativ pr growths from the young 
sporophyte of Polypodium trinddas, Bull. .. “Club 48: 203-205. f. 1-3. 1921- 
