28 HUBER. [Vol. XVI. 



My reason for presenting the following research may, if such 

 reason be required, be briefly stated as follows : 



It seemed to me desirable to confirm some of the observa- 

 tions previously made, with what seemed to me somewhat im- 

 proved histological methods, hoping at the same time to throw 

 new light on some of the still disputed questions, and especially 

 to broaden our knowledge of these structures by systematically 

 investigating the sympathetic ganglia of types from the several 

 classes of vertebrates, using in each case the same methods. 



During the investigation, material was obtained from the 

 following vertebrates : 



Fishes. 



Ambloplites nipestris (Raf.), rock bass. 



Micropterus dolomieu (Raf.), small-mouth black bass. 



Perca flavescens (Mitch.), perch. 



Amphibia. 



Rana Catesbiana. Rana Hal. 



Reptilia. 



Chrysemys picta. Chelydra serpentina. 

 Emys meleagaris. 



Birds. 



Gallus domesticus — chicken. 

 Mammalia. 



Dog, cat, rabbit, and guinea pig. 



The subject-matter to be discussed in this research will be 

 taken up as follows : 



i) A brief discussion of the methods used. 



2) A descriptive account of the results obtained in the prep- 

 arations made from each of the above classes of vertebrates, 

 in the order above named, with the literature bearing on the 

 subject. 



3) The general conclusions which may be drawn. 



Method. 



In the earlier portion of this work both the chrome silver 

 and the methylene blue method were used ; the former was, 

 however, soon discarded, owing to its greater uncertainty ; 



